Coding
Part:BBa_K2546004:Design
Designed by: Tsu-Wang Sun Group: iGEM18_NCHU_Taichung (2018-10-03)
pME60C shuttle vector
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 5379
Illegal SpeI site found at 6471 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 5379
Illegal NheI site found at 2246
Illegal SpeI site found at 6471
Illegal NotI site found at 1547
Illegal NotI site found at 2837
Illegal NotI site found at 5385
Illegal NotI site found at 6478 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 5379
Illegal BamHI site found at 4230
Illegal XhoI site found at 4363
Illegal XhoI site found at 5255 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 5379
Illegal SpeI site found at 6471 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 5379
Illegal XbaI site found at 5394
Illegal SpeI site found at 6471
Illegal NgoMIV site found at 1464
Illegal NgoMIV site found at 2445
Illegal NgoMIV site found at 2590
Illegal NgoMIV site found at 2594
Illegal NgoMIV site found at 2801
Illegal NgoMIV site found at 3023
Illegal NgoMIV site found at 3269
Illegal AgeI site found at 216
Illegal AgeI site found at 540
Illegal AgeI site found at 1752
Illegal AgeI site found at 6181
Illegal AgeI site found at 6293 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1245
Illegal BsaI.rc site found at 1849
Illegal SapI site found at 1620
Design Notes
asd
Source
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